2005 Program
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2005 Participant:Carmen Gacchina


Project Title:
The Effects of P-selectin Density on Nano-scale Patterned
Surfaces for Modeling Leukocyte-Endothelial Cell Interactions.

Faculty Advisor: Brian Helmke

 
Carmen making microscopic analysis of bovine aortic endothelial cells, checking
for confluency prior to "splitting."


Project Summary:

This project utilizes both materials science and biomedical engineering expertise. Patterned Au/Al2O3 substrates are designed using sputtering, anodization, electrochemical plating, and backsputtering. Varying amounts of P-selectin are introduced and through chemical means these are attached to the gold pattern. Finally, flow experiments model leukocyte rolling on the patterned surface. This research will guide the design of cell phenotype for regenerative medicine and tissue engineering in vivo.
A thin film of gold imprinted by a nano-patterned surface
for adhesion of fibronectin protein (in petri dish).
Endothelial cells will then be introduced to the surface,
where these adhere to the protein.
 
 
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